Preclinical study of immunogenicity of adjuvanted quadrivalent subunit influenza vaccine

Background. Preventive vaccination is a vitally important strategic aspect of protection the population against severe effects influenza epidemics. The priority attention given to development effective tetravalent vaccines containing antigens two A lineages (H1N1, H3N2) and B (Victoria Yamagata) in combination with immunoadjuvants.The aim work was conduct preclinical study immunogenicity protective efficacy innovative subunit vaccine viruses as well corpuscular adjuvant.Materials methods. conducted using female BALB/c mice. monovalent intermediate combined betulin adjuvant were injected intraperitoneally times at 14-day interval. immunogenic activity measured by hemagglutination inhibition assay. assessed changes viral load, body weight survival rates mouse model fatal H1N1 virus infection.Results. mice vaccinated adjuvanted quadrivalent produced antibodies all four included vaccine; mean antibody titers assay above 1 : 40. second-dose induced significant increase levels viruses. dose 5 µg each antigen 200 provided 100% rate significantly decreased lung (more than 3 lg TCID50) pneumonia.Conclusion. betulin-based demonstrates high laboratory provides pneumonia caused subtype H1N1.